Attempt to isolate infectious agent from bone-marrow of patients with multiple sclerosis.
نویسندگان
چکیده
Bone-marrow was aspirated into 10 ml Eagle's minimal essential medium (EMEM) containing preservative-free heparin. After washing, the samples were divided into four parts; A for tube cultures,. B for coverslip cultures in multidish wells; C was placed on a 'Ficoll'-'Hypaque' gradient to obtain free mononuclear cells, and D was frozen in liquid nitrogen in cryopreservative medium containing 1% dimethyl sulphoxide.
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ورودعنوان ژورنال:
- Lancet
دوره 2 8139 شماره
صفحات -
تاریخ انتشار 1979